Hedgehog signaling pathway mediated Gpc1 regulation on the proliferation of neuroglioma cells

Abstract:

Objective：To explore the effects of altered expression of Gpc1 expression on the proliferation of glioma cells and to address the potential role of hedgehog signaling pathway in the mediation of Gpc1 action. Methods：One cell line having relatively high activa－tion in Hedgehog signaling pathway and higher Gpc1 expression from four glioma cell lines was selected via Western blot. Gpc1-RNAi stable cell line was constructed to detect the changes of Hedgehog pathway component. The activity of Hedgehog via luciferase reporter gene after altering Gpc1 expression was detected. Gpc1-RNAi stable cell line proliferation viability was detected by MTT. Results：The Hedgehog pathway was suppressed（F=8 407.34，P=0.000） and glioma cell viability was restricted（F=27.68，P=0.000） after knock－ing down Gpc1 expeession. Conclusion：Glioma cell proliferation can be regulated by Gpc1 via Hedgehog pathway