Influence of docetaxel-loaded lipid microbubbles combined with ultrasound targeted microbubbles destruction on proliferation and apoptosis of hepatocellular carcinoma cell

Abstract:

Objective：To study the influence of docetaxel-loaded lipid microbubbles（LDLM） combined with ultrasound targeted mi－crobubbles destruction（UTMD） on proliferation and apoptosis of SMMC-7721 cells. Methods：Suitable concentration of docetaxel was chosen. SMMC-7721 cells were cultured in vitro and divided into 6 groups. Proliferating inhibition rate，ultrastructural change，apop－totic rate and cell cycle distribution were compared among all groups. Results：The proliferating inhibition rate and apoptotic rate of LDLM+US group were the highest compared with that of other groups（P=0.000，P=0.000）. Great amount of apoptotic cells were observed in LDLM+US group by electron microscope and flow cytometry. Proportion of cells at G0/G1 phase was decreased obviously（0.79±0.27）%（P=0.000） while that at G2/M phase was obviously increased（90.54±0.48）%（P=0.000）. Conclusion：LDLM combined with UTMD could inhibit the proliferation and promote the apoptosis of SMMC-7721 cells，which provide evidence for the further study of its mechanisms in protein and gene level.