Expression of PRDX2 in colorectal neoplasms and its mechanism

Abstract:

Objective：To investigate the expression of PRDX2 in histological samples of colorectal neoplasm and the normal tissues and to analyze its mechanism. Methods：The expression of PRDX2 was detected by immunohistochemistry and Western blot. In colon cancer cell SW480，interacting proteins with PRDX2 was detected by immunoprecipitation and HPLC-CHIP-MS/MS. Results：Im－munohistochemistry indicated that PRDX2 protein was expressed mainly in the cytoplasm. The expression of PRDX2 was weaker in normal colorectal tissues（21.88%，7/32） than in carcinomas tissue（71.88%，23/32）（P=0.001）. Western blot analysis revealed that the expression of PRDX2 protein was significantly higher in carcinomas than in control colorectal tissues（P<0.01）. In SW480 cells，17 proteins were obtained from immunoprecipitation using specific antibodies against PRDX2，9 of them were regulated by c-Myc gene. Conclusion：PRDX2 play roles in the development and metastasis of colorectal cancer. PRDX2 might be associated with c-Myc gene regulatory pathway.