Dynamic pathological changes in the brain of female APP/PS1 double transgenic mice with different ages

Abstract:

Objective：To discuss the dynamic pathological changes in the brain of female Alzheimer’s disease（AD） mice with differ－ent ages. Methods：Totally 24 APP/PS1 double transgenic mice with AD of 3，8，12，16-month-age were chosen（6 mice for each age group）. Light microscopic observation was performed after Paraffin embedding，sectioning and immunohistochemical staining. Results Immunohistochemical results showed that，amyloidβ peptide（Aβ） and senile plaque（SP） can be occasionally observed. With the in－crease of the age，the volume and number of SP were increased（M3=0.600±0.235，M8=8.933±1.730，M12=28.470±1.841，M16=94.130±4.287；P1=0.025，P2=0.000，P3=0.000）（M3，M8，M12，M16 stands for 3，8，12，16 months respectively；P1，P2，P3 stands for the comparison of P value between M3 and M8，M8 and M12，M12 and M16）. Accordingly，NeuN positive cells in the brain of mice were in a declined trend（M3=1 919.00±58.21，M8=1 717.00±65.83，M12=1 689.00±45.66，M16=1 529.00±45.36，P1=0.011，P2=0.712，P3=0.043），glial fibrillary acidic protein-positive cells were increased（M3=9.2±6.7，M8=1 053.0±171.3，M12=2 058.0±210.8，M16=3 283.0±240.3；P1=0.000，P2=0.000，P3=0.000） and β-site APP cleaving enzyme 1 positive cells were increased（M3=2 183.00±85.43，M8=3 466.00±142.50，M12=4 555.00±83.23，M16=5 237.00±133.40；P1=0.000，P2=0.000，P3=0.000）. Similar results were achieved by Western blot. Conclusion：Female APP/PS1 double transgenic mice display typical AD pathological changes from the age of 3 months to 16 months. The pathological changes grad－ually increase with increasing of age，without reversal.