Mechanism of enterovirus 71 entering into human neuroblastoma cells

Abstract:

Objective：To research the mechanism of enterovirus type 71（EV71） entering into human neuroblastoma SK-N-SH cells. Methods：The clinical EV71 isolates were inoculated from hu－man rhabdomyosarcoma（RD） cells for amplifying and purify－ing virus. MTT assay was conducted to detect the effect of dif－ferent virus endocytosis blockers on inhibition of SK-N-SH cells. The impact of target cells SK-N-SH on EV71 mRNA expression was validated by TaqMan real-time PCR after treating by specific chemical blockers. Results：EV71 viruses were success－fully amplified in RD cells and the virus titer was 1×105 TCID50. With the increase of drug concentration gradient，the growth rate of SK-N-SH cell was inhibited. TaqMan real-time PCR showed that the expression of EV71 mRNA was inhibited by pretreatment of chlorpromazine（CPZ）（Ρ<0.05） and nystatin（NT） exerted little influence（Ρ>0.05）. Conclusion：EV71 invading into SK-N-SH cells by clathrin-dependent endocytosis is initially speculated.