Effect of focused ultrasound on neuroglioma model of zebrafish

Abstract:

Objective：To explore the treatment effect of focused ultrasound on neuroglioma model of zebrafish. Methods：Transgenic zebrafishes（fli：GFP） were randomly divided into control group，U87 group and U87+HIFU group. Neuroglioma cells（U87-RFP） were injected into U87 group and U87+HIFU group after 2 d fertilization and focused ultrasound treatment was performed in U87+HIFU group after 24 h. The tumor size and the vessels were observed using fluorescence microscopy on the 1st d and the 2nd d after the treatment. The mRNA expression of vascular endothelial growth factor（VEGF） Aa and vascular endothelial growth factor receptor 2 VEGFR2 were evaluated by qPCR. Results：In U87 group，a large number of neuroglioma cells and the newly formed vessels induced by neuroglioma were easily observed in zebrafish’s yolk ball，while after focused ultrasound treatment，neuroglioma cells and the ves－sels were significantly decreased. qPCR suggested that the mRNA of VEGF Aa and VEGFR2 were highly expressed in U87 group（VEGF Aa：Fli：1.01±0.13 vs. U87：1.45±0.10，P=0.002；VEGFR2：Fli：1.01±0.12 vs. U87：1.41±0.07，P=0.001），but after focused ultrasound treatment，the expression of VEGF Aa and VEGFR2 were inhibited（VEGF Aa：U87+HIFU：1.14±0.05 vs. U87：1.45±0.10，P=0.009；VEGFR2：U87+HIFU：1.16±0.06 vs. U87：1.41±0.07，P=0.012）. Conclusion：Focused ultrasound treatment can effectively kill the neuroglioma cells and decrease the tumor angiogenesis，which shows a positive treat effect on neuroglioma.