Effect of activator protein 2α on BMP9-induced osteogenesis differentiation of C3H10 and its mechanism

Abstract:

Objective：To research the effect of activator protein 2α on the process of bone morphogenetic protein 9（BMP9）-induced osteogenesis differentiation of mouse mesenchymal stem cells（C3H10） and its mechanism. Methods：C3H10 cells were infected with Ad-BMP9. Adenovirus-mediated dominant negative mutants Ad-dnAP2α-△bHLH and Ad-dnAP2α-△TAD were used to inhibit AP2α transcriptional activity. RT-PCR，real-time PCR and Western blot methods were used to detect the expression of AP2α，BMP9 and osteogenetic related markers. Alkaline phosphatase reading/staining and alizarin red staining were performed to detect the status of osteogenetic differentiation. Results：Over-expression of BMP9 could accelerate osteogenesis differentiation but did not affect the expression of AP2α. AP2α dominant negative mutants could inhibit the BMP9-induced increase of osteogenesis related markers osteocalcin（OC），osteopontin（OPN）and osteoprotegerin（OPG）expression（OC：F=56.929，P=0.000；OPN：F=54.789，P=0.000；OPG：F=159.451，P=0.000）and reduce the alkaline phosphatase activity（F=69.776，P=0.000） and red staining of calcium salt deposi－tion. Conclusion：AP2α transcription activity might regulate the process of BMP9-induced osteogenesis differentiation.