Expression of Taenia solium TSO45W-4B gene in bifidobacterium longum

Abstract:

Objective：To observe the expression of the gene of TSO45W-4B of Taenia solium in bifidobacterium longum（B.longum） on the basis of successful construction of the escherichia coli（E.coil）-bifidobacterium shuttle plasmid pGEX-TSO45W-4B of Taenia solium. Methods：The shuttle expression plasmid pGEX-TSO45W-4B of Taenia solium was electroporated into B.longum. After induc－tion with isopropyl-β-d-thiogalactoside，the expression of the recombinant protein was identified by sodium dodecyl sulfatepolyacry－lamide gel electrophoresis（SDS-PAGE） and Western blot. Results：Restriction analysis，polymerase chain reaction and sequencing showed that the recombinant plasmid pGEX-TSO45W-4B was successfully transformed into B.longum. As demonstrated by SDS-PAGE，the relative molecular mass（Mr） of the expressed recombinant protein was approximately 40 kD，and the purity of the recom－binant protein was 85% after purification withglutathione S-transferase affinity chromatography. The rabbit antiserum of TSO45W-4B，cysticercosis swine serum and cysticercosis patients serum could bind to the recombinant protein in Western blot assay. Conclusion：The gene of TSO45W-4B of Taenia solium could be expressed in B.longum and the expressed recombinant protein shows specific antigenicity.