Expression of TRIM28 in non-small cell lung cancer and its effect on the growth of lung adenocarcinoma PAa cell

Abstract:

Objective：To identify the expression of TRIM28 in non-small cell lung cancer（NSCLC），to study the effect of TRIM28 gene on the growth of non-small cell lung cancer. Methods：Immunohistochemistry was used to detect the expression of TRIM28 in clinical tissue of non-small cell lung cancer patients. After TRIM28 gene was inhibited by RNA interference assay，soft agar assay and MTT cell proliferation assay were used to observe the growth and proliferation of lung adenocarcinoma PAa，and flow cytometry was used to an－alyze the cell cycle. Results：TRIM28 positive staining was observed in 24 of 48（50.0%）non-small cell lung cancer tissue samples，whereas no staining was observed in any of the normal portions of the same tissues（ ?字2=27.034，P=0.000）. TRIM28 knockdown resulted in a decrease in cell proliferation and a significant population in G1/S fraction. Conclusion：TRIM28 expresses highly in non-small cell lung cancer tissues. The growth of lung adenocarcinoma PAa transfected with TRIM28 siRNA is inhibited suggesting that TRIM28 may be new therapy target for NSCLC.