Effect of nerve growth factor and K252α and UO126 on proliferation of granulosa cells and expression of proliferating cell nuclear antigen

Abstract:

Objective：To observe the mouse ovarian preantral granulose cells proliferation and expression of proliferating cell nuclear antigen（PCNA） in order to investigate the mechanism of nerve growth factor（NGF） and K252α and UO126. Methods：The mouse ovarian preantral granulose cells were treated with NGF and inhibitors K252α and UO126 in vitro. Cell proliferation was measured by cell counting Kit-8（CCK-8） assay and change in the expression of PCNA was detected by Western blot. Results：The data showed that the proliferation of granulose cells was significantly increased in NGF group（A value=1.58） than in control group（A value =1.31）（PNGF＝0.000）. The proliferation of granulose cells was significantly decreased in K252α group（A value=1.21），NGF+K252α group（A value=1.19），NGF+UO126 group（A value=1.14） than in control group（PK252α＝0.001，PNGF+K252α＝0.001，PNGF+UO126＝0.000） after applying K252α and UO126. Relative expression of PCNA was higher in NGF group（5.21） than in control group（1.65）（PNGF＝0.000）. Relative expression of PCNA was respectively 1.48，0.84，0.41 in K252α group，NGF+K252α group and NGF+UO126 group，with statistical differences compared with that in control group（PK252α＝0.02，PNGF+K252α＝0.000，PNGF+UO126＝0.000） and that in NGF group（PK252α＝0.000，PNGF+K252α＝0.000，PNGF+UO126＝0.000）. Conclusion：NGF could significantly promote the proliferation of granulose cell and at the same time induce the expression of PCNA. However，the inhibitors suppress the proliferation of granulose cell，which might be associated with the declining expression of PCNA.