Effect of curcumin combined FOLFOX regimen on proliferation inhibition and apoptosis-inducing of gastric carcinoma BGC-823

Abstract:

Objective：To investigate the effect of curcumin combined folinic acid fluorouracil oxaliplatin（FOLFOX） on proliferation inhibition and apoptosis-inducing of the gastric cell line BGC-823，and to explore its possible mechanisms. Methods：Gastric cancer cell line BGC-823 cells were divided into blank control group，curcumin group（10 μmol/L），FOLFOX group（5-FU 0.1 mmol/L+Oxaliplatin 5 mmol/L）] and curcumin combined FOLFOX group. CCK-8 was used to detect the cell proliferation. Cell apoptosis was observed by Hoechst staining. The mRNA and protein expressions of Bcl-2 and Bax were measured by real-time quantitative PCR and Western blot. Caspase-3 vitality was tested by Caspase-3 test kit. Results：The proliferation inhibition rate of BCG-823 cell in curcumin combined FOLFOX group was significantly higher than those in other groups（P<0.05）. Hoechst staining showed that apoptotic cells in medicated groups were higher than those in control group，being the most obvious in FOLFOX group. Compared with those in the control group，the expressions of Bcl-2 mRNA，Bcl-2 protein were decreased in other groups，while the expressions of Bax mRNA，Bax proteins were increased with statistically significant differences（P<0.05）. Caspase-3 activity was also increased significantly in the medicated groups，especially in curcumin combined FOLFOX group（P<0.05）. Conclusion：Curcumin combined FOLFOX could inhibit the proliferation and induce apoptosis of GC-823 cells，which was related with the activation Bax / inactivation Bcl-2 and the activation of Caspase-3.