Expression and significance of adiponectin，resistin and Apelin in hepatocyte steatosis

Abstract:

Objective：To explore the expression and significance of adipocytokines adiponectin，resistin and Apelin in hepatocyte steatosis induced by oleic acid. Methods：Routinely cultured human L02 cells were used as controls；L02 cells were induced by oleic acid for 24，48，72 h respectively to establish nonalcoholic hepatocyte steatosis models（model groups）. Lipid droplets in cells were observed by oil red staining and the levels of triglyceride were measured with analyzed kit. The expressions of mRNA and protein of adiponectin，resietin and Apelin were measured by qPCR and Western blot. Results：Oil red O staining showed that hepatocyte steato－sis occurred at 24 h after L02 cells being treated with oleic acid and hepatocyte steatosis increased greatly at 72 h. Triglyceride in hep－atocytes of model groups also increased compared with that of control group. In model groups，the expression levels of adiponctin mRNA and protein decreased at 24 h and declined remarkably at 72 h，compared with those of control groups. The expression levels of resistin mRNA increased at 24 h and elevated remarkably at 72 h in model groups，compared with those in control group. The expression levels of resistin protein increased at 48 h and elevated remarkably at 72 h in model groups，compared with those in control group. The ex－pression levels of Apelin mRNA and protein increased at 24 h and elevated remarkably at 72 h in model groups，compared with those in control group. Conclusion：The expressions of adiponectin are down-regulated while those of resistin and Apelin are up-regulated in hepatocyte steatosis model，indicating the regulation of adipocytokines. Adiponectin resistin and Apelin may be jointly involved in hepatocyte steatosis.