Comparison on two types of external drainage in pig pancreaticoduodenal transplantation

Abstract:

Objective：To establish two types of animal model of pancreatoduodenal transplantation in pigs with enteric drainage（ED） and stomachic drainage（SD），to explore a more physiological operation method with fewer complications. Methods：Twenty-four close group white pigs were divided into ED group and SD group randomly；the pigs in each group were randomly subdivided into donor and receptor groups for pancreatoduodenal transplantation with ED or SD. The levels of blood glucose，insulin，glucagon and amylase were measured at the following time：24 h before operation，before the start of anesthesia，30 min after the pancreas removed，after the reperfusion，30 min and 12 h after the pancreatoduodenal transplantation. Postoperative complications and graft function were observed. Results：The success rate of surgery was 100%. The operation time was slightly longer in SD group than in ED group，but without sta－tistically significances（P=0.551）. Complications occurred in 2 cases in ED group：one was graft thrombosis and the other one was pancreatic fistula. One case of anastomotic fistula occurred in SD group. No significant difference was found between the two groups in blood glucose，insulin and serum amylase in each period. The levels of glucagon between the two groups were statistically different（P=0.024 4），but the result may be false positive. The differences between groups were statistically significant. Conclusion：Pancreato－duodenal transplantation in pigs with ED and SD is feasible. SD can facilitate postoperative pathological biopsy to determine the im－mune rejection. Numbers of clinical studies are needed to deter－mine the complications，immune rejection，survival rate，graft survival of the two operative methods.