miR1934，an adiponectin specially-regulated microRNA，is downregulated in adipose tissues of obese mice

Abstract:

Objective：Mice over-expressing ApN specifically in adipose tissues（AT，ApN-Over） and the mice knocking-out ApN （ApN-KO） were used to identify microRNAs（miRNAs） targeted by ApN in AT. Methods：Male ApN-Over mice，ApN-KO mice and C57/Bl6J mice fed with high-fat diet（HFD） were used. miRNA expression profiling was performed by using microRNA arrays. The expressions of miRNAs and adipokines were quantified by real-time PCR. The gene targets of miR1934 were predicted by bioinfor－matic data base. Results：There were 12 miRNAs differently expressed between AT of ApN-Overex mice and wild-type mice. By PCR validation，the expression of miR532-5p was down-regulated，while that of miR883b-5p and miR1934 was up-regulated in AT of ApN-Over mice. Moreover，the expression of miR1934 in AT of ApN-KO mice was down-regulated，exhibiting a reverse expression pattern compared to ApN-Over mice. The expression of miR1934 was significantly lower in epididymal AT of HFD-induced obese mice than that in chow diet-fed mice（1.00±0.22 vs. 0.54±0.13，P=0.04）. The predicted gene targets of miR-1934，serine/threonine kinase 3（STK3） and chemokine（C-C motif） ligand（CCL19），were annotated to be involved in Jun N-terminal kinases（JNK） signal－ing pathway. Conclusion：We identified a novel miRNA，miR-1934，which was up-regulated by ApN in AT in vivo. The expression of miR1934 was decreased in visceral AT of obese mice. The possible roles of miR-1934 may be involved in the JNK signaling pathway. This novel miRNA may open new therapeutic perspectives for controlling the pro-inflammatory state of obese AT.