Expression of GAPDH and β-actin during osteogenic differentiation of BMP9-induced mesenchymal stem cell

Abstract:

Objective：To observe the changes of glceraldehyde-3-phosphate dehydrogenase（GAPDH） and β-actin gene expression during bone marrow mesenchymal stem cells（MSCs） differentiation to the osteogenic lineage induced by bone morphogenetic proteins 9 in order to select more suitable reference genes. Methods：Alkaline phosphatase（ALP） was used as a marker to identify BMP9-in－duced MSCs’ osteogenic differentiation；and mRNA and protein of GAPDH and β-actin were detected by real-time PCR and West－ern blot respectively on 0，1，3，5，7 d during MSCs’ osteogenic differentiation. Results：（1）mRNA expression of β-actin was signifi－cantly increased during osteogenic differentiation；there were significant differences in mRNA expression of β-actin between d0 and d5；no apparent change was observed in GAPDH. Results showed that the protein level of β-actin was increased gradually espe－cially on d5 and d7，while no apparent change was observed in GAPDH. Conclusion：During BMP9 induced MSCs’ osteogenic dif－ferentiation，GAPDH is more suitable than β-actin as internal reference gene.