Effects of extra and intracellular gelsolin levels on polymorphonuclear neutrophil chemotaxis

Abstract:

Objective：To investigate the effects of intracellular or extracellular gelsolin level on N-formylmethionyl-leucyl phenylala－nine（fMLP） induced rat polymorphonuclear neutrophil（PMN） chemotaxis. Methods：PMN neutrophils were isolated from rat venous blood. Freshly prepared PMN suspension was seeded to the upper chamber of Transwell culture system and recombinant gelsolin was added to the upper or lower chamber to investigate the effect of gelsolin on PMN chemotaxis. Recombinant gelsolin or anti-gelsolin antibodies were transfected into PMN with electroporation method and the chemotaxis function was observed respectively. Cell counts in lower chamber were recorded in real time. Results：There was no obvious difference in PMN chemotaxis，regardless of gelsolin was added in the upper or lower chamber or transfected into cytoplasm. However，the rate of chemotaxis and chemotaxis index of PMN electrotransfected with anti-gelsolin antibodies was decreased significantly. Conclusion：Changing intracellular or extracellular gelsolin level exerts on effect on fMLP induced PMN chemotaxis；but electrotransfection with anti-gelsolin antibodies intracellular inhibits fMLP-induced rat PMN chemotaxis.