Improving the diagnosis of acute leukemia by applying FISH combined with G-banding chromosome analysis

Abstract:

Objective：To investigate the diagnosis of acute leukemia by applying FISH combined with G-banding chromosome analysis. Methods：Bone marrow specimens were collected from 410 children with acute leukemia（AL） from 2011 January to 2012 December. The cells were cultured and chromosome analysis was made by using G-banding method. Among them，57 AL cases were simultane－ously examined by FISH with their corresponding alteration genes. Results：The chromosomal abnormal rate was 41%（168/410） in 410 AL cases. Among the 57 AL cases simultaneously examined with FISH and chromosome analysis，the chromosome abnormal rate of G-banding method was 52.6%（30/57）. However，the abnormal rate of FISH was 68.4%（39/57）. The consistency of G-banding chromo－some analysis and FISH was 82.5%. Conclusion：FISH is superior to G-banding chromosome analysis when analyzing chromosome aberration in acute leukemia. Applying FISH combined with G-banding could improve the accurate and reliable results for clini－cal diagnosis of acute leukemia.