Application of Akt inhibitor in the oxygen-induced retinopathy

Abstract:

Objective：To examine whether Akt inhibitor treatment during the phase of oxygen-induced retinopathy helps to prevent retinal neovascularization. Methods：Seven-day-old C56BL/6J mice were exposed to（75±2）% oxygen for 5 d，then returned to the room air to induce rentinal neovascularization. Fourteen mice were equally randomized into control group and experiment group. On P12，1.5 μl Akt inhibitor was injected into vitreous cavity of mice in experiment group and PBS was injected into mice in control group. Retinal neovascularization was analyzed with FITC-Dextran and histological analysis on P17. Results：Mice treated with Akt inhibitor had a reduced pathologic neovascular tufts and fluorescence leakage compared with those of PBS controls. For the area of capillary-free，no difference was seen between experiment group and control group. The histological analysis showed that neovascular nuclei protruding into viteous cavity were decreased in experiment group compared with those in control group. Conclusion：Akt inhibitor treatment protects the retina from vessel pathological proliferation，but cannot protect the retina from vessel loss.