Role of PAR2 in proliferation of injured spinal cord astrocytes of rats

Abstract:

Objective：To explore the role of protease activated receptor2（PAR2） in proliferation of injured spinal cord astrocytes of rats. Methods：High purity spinal cord astrocytes of rats were achieved from primary culture（purity>98%） and the injured astrocytes model in vitro was established. Injured astrocytes were treated by normal saline（injured group），PAR2 inhibitor FSLLRY-NH2（FS－LLRY-NH2 group） and PAR2 antibody Anti-PAR2 antibody（Anti-PAR2 antibody group） immediately. Primary culture cells without any treatment were included in control group. The expression levels of GFAP and Vimentin，marker proteins of astrocytes，were detected using the immunofluorescence staining and Western blot after injured astrocytes model being established and treated. Proliferative ac－tivity of astrocytes was detected by CCK-8 after injured astrocytes model being established and treated. Results：At 24 h and 72 h after injured astrocytes model being established，the expression levels of GFAP and Vimentin were significantly higher in injured group compared with those in control group，FSLLRY-NH2 group and Anti-PAR2 antibody group；proliferative activity of astrocytes was also significantly higher in injured group compared with that in control group，FSLLRY-NH2 group and Anti-PAR2 antibody group at 12，24，72 h after injured astrocytes model being established（P<0.05）. Conclusion：Blocking PAR2 can inhibit proliferation of injured spinal cord astrocytes of rats.