Association of brain-derived neurotrophic factor gene polymorphisms with vascular cognitive impairment in Chongqing

Abstract:

Objective：To investigate the association of brain-derived neurotrophic factor（BDNF） gene polymorphisms with vascular cognitive impairment（VCI） in Chongqing. Methods：Totally 60 patients with VCI（VCI group） 99 health（normal control group） were chosen simultaneously. The genotypes and alleles frequencies of BDNF gene were analyzed by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）. The software SHEsis was used to analyze the linkage disequilibrium and the haplotypes between the two groups. Results：G11757C genotypes frequencies between VCI group（GG（30.4%），GC（53.6%），CC（16.1%）） and normal control group（GG（30.9%），GC（47.4%），CC（21.6%））showed no significant difference and alleles frequencies between VCI group（G（57.1%），C（42.9%））and normal control group（G（54.6%），C（45.4%）） also showed no significant difference（ χ2=0.837，P=0.658； χ2=0.180，P=0.671）. G196A genotypes frequencies between VCI group（AA（23.3%），AG（51.7%），GG（25.0%）） and normal control group（AA（22.2%），AG（46.5%），GG（31.3%））showed no significant difference and alleles frequencies between VCI group（A（49.2%），G（50.8%）） and normal control group（A（45.5%），G（54.5%）） also showed no significant difference（ χ2=0.744，P=0.689； χ2=0.413，P=0.520）. C270T genotypes frequencies（CC（8.30%），CT（56.7%），TT（35.0%）vs. CC（24.2%），CT（50.5%），TT（25.3%））and alleles frequencies（C（36.7%），T（63.3%） vs. C（49.5%），T（50.5%）） showed significant differences between VCI group and normal control group（ χ2=6.68，P=0.035； χ2=4.98，P=0.026）. And these were significant differences in the frequency haplotype GGT（Fisher’s P=0.025） between VCI group and normal control group. Con－clusion：The polymorphisms of BDNF gene may be related to VCI in Han population in Chongqing.