Effect of MUC1 cytoplasmic domain inhibitor on the radiosensitivity of lung cancer cells

Abstract:

Objective：To explore the radiosensitive effect of GO-201 on lung cancer cell line A549 cultured in vitro. Methods：MTT assay was used to detect the growth inhibition rate；the radiosensitive effect was detected by clone formation test. Cell survival cure was fitted by multitarget one-hit model and Dq，D0，SF2，sensitizing enhancing ratio（SER） was calculated. Additionally，flowcytometry and Western blot were also conducted to observe the influence of GO-201 and radiation on cell reactive oxygen species and the expression of manganese superoxide dismutase（MnSOD） protein. Results：GO-201 could inhibit the proliferation of lung cancer cell（IC50 value were 30.79 ?滋mol/L，23.32 ?滋mol/L，13.60 ?滋mol/L after 24，48，72 h） and enhance radiosensitivity of A549 cells；the SER was 1.14. Reactive oxygen level of A549 cell was increased（F=12.019，P=0.008） and the expression of MnSOD protein was decreased（F=5.465，P=0.048） in combination group than in control group. Conclusion：Inhibiting MUC1 cytoplasmic domain can improve the radiosensi-tivity of lung cancer cell.