Effect of JAZF1 over-expression on gluconeogenesis and insulin signal pathway

Abstract:

Objective：To establish juxtaposed with another zinc finger gene 1（JAZF1）-transgenic mice model on the basic of cell re－search in vitro and to explore the effect of JAZF1 over-expression on gluconeogenesis and insulin signal pathway. Methods：The pre－pared JAZF1 gene fragment was microinjected into one-cell embryos of mice，then the JAZF1-transgenic mice were generated and i－dentified by standard procedures. Eight-week-old Tg-JAZF1（Tg） mice and wild type（WT） mice were randomly divided into 4 groups：standard diet（SD）-WT group（n=6），SD-Tg group（n=6），high fat diet（HFD）-WT group（n=6） and HFD-Tg group（n=6）. Phospho－enolpyruvate carboxykinase and glucose-6-phosphatase in liver were detected by PCR and Western blot after 12 weeks. The phos－phorylation of insulin signal protein was measured by Western blot. Results：JAZF1 mRNA and protein expressions in liver，muscle and adipose tissue were elevated significantly in SD-Tg mice than in SD-WT mice（P<0.05）.The expressions of gluconeogenesis gene in Tg mice were dramatically decreased compared with those of WT mice under the condition of SD or HFD（P<0.05）. The phospho－rylation of insulin signal protein in liver，muscle and adipose tissue were increased in Tg mice than in WT mice（P<0.05）. Conclusion：Transgenic mice model has been generated successfully. JAZF1 overexpression inhibit the expression of gluconeogenesis gene and also improve the insulin sensitivity of liver，muscle and adipose tis－sue.