Effects of celecoxib on gastric emptying in diabetic gastroparesis rat

Abstract:

Objective：To investigate the effects of celecoxib on gastric emptying in diabetic gastroparesis rat. Methods：Male SD rats were divided into normal control group，experimental group. Diabetes was induced by intraperitoneal injection of streptozotocin（STZ，50 mg/kg） and diabetic gastroparesis（DGP） was induced by feeding with a high-carbohydrate/high-fat diet. The normal control group（NC group，n=7） was injected with equivalent phosphate buffer solution and fed with standard diet. After 8 weeks，the diabetic rats were randomly divided into two groups ，namely diabetic gastroparesis group（DGP group，n=7） and celecoxib-treated group（CE group，n=7）. The diabetic rats were orally administered with celecoxib [40 mg/（kg·d）] for 3 weeks. Gastric emptying was detected through semisolid method. The mRNA expression of COX-2 was measured by quantitative reverse transcription-PCR. COX-2 protein level was assessed by Western blot. Moreover，prostaglandin E2（PGE2） was quantified by ELISA. The ultrastructural of gastric muscle was evaluated by transmission electron microscopy. Results：Celecoxib significantly improved the gastric emptying of DGP rat（F=64.123，P=0.000）. Meanwhile，celecoxib decreased the expression of COX-2 mRNA（F=9.928，P=0.005） and COX-2 protein（F=28.543，P=0.001） and PGE2（F=55.378，P=0.000） of gastric antral smooth muscle. There were swollen mitochondria and vacuolation in the gastric muscle of DGP rats. Only several myelin figures emerged in celecoxib-treated group. Conclusion：Celecoxib may ame－liorate gastric emptying of DGP rat through inhibition of the COX-2/PGE2 pathway.