PIN1 overexpression activates JNK signal pathway and upregulates CyclinD1 in nasopharyngeal epithelium cell

Abstract:

Objective：To investigate the cancer signal pathways which prolylisomerase（PIN1） may regulate. Methods：PIN1 overex－pression NP69 cell line was established by using the leti-viral system and the PIN1 expression was proved by q-PCR and Western blot. Then PIN1 regulated cancer signal pathways were analyzed by using cancer 10-pathway reporter assay and Western blot. Results：PIN1 over expression in nasopharyngeal epithelia cell may activate a serial cancer signal pathways including MAPK/JNK signal pathway，NF-κB signal pathway and Myc signal pathway. Comparing with those control group，Western blot results showed NP69-Lenti-PIN1 group had higher quantity expressions in c-Jun，c-Jun Ser63，c-JunSer73 and CyclinD1（t=-7.295，P=0.002）. Conclusion：The over-expression of PIN1 up-regulates CyclinD1 expression，activates the MAPK/JNK pathway，and increases phosphorylation of c-Jun at Ser63 and Ser73. Therefore PIN1 may contribute to the tumorigenesis of nasopharyngeal carcinoma.