Borna disease virus encoded phosphoprotein down-regulates the proliferation of human oligodendrocytes by regulating miR-134

Abstract:

Objective：To investigate the proliferation of oligodendrocyte（OL） cells induced by miR-134 in Borna disease virus（BDV） infection. Methods：The expression levels of miR-134 in OL and OL/BDV were detected by real-time PCR. BDV phosphoprotein （P24） or nucleoprotein（P40） gene expression vector was used to transfect OL cells respectively；the effects of BDV P24 and P40 pro－teins on the expression of miR-134 were detected by real-time PCR. The OL cells proliferation ability was investigated by flow cytom－etry（FCM），along with miR-134 transfection and BDV infection. Results：There were significant differences in expression of miR-134 between OL and OL/BDV cells by real-time RT-PCR（P=0.000）. The expression of miR-134 was significantly increased by BDV P24 overexpression（P=0.000），but not by BDV P40（P=0.139）. Both overexpression of miR-134（P=0.016） and BDV infection（P=0.001） down-regulated the proliferation of OL cells. Conclusion：BDV P24 protein down-regulates the proliferation of OL cells by up-regu－lating miR-134.