Analysis of the interaction of Beta-Arrestin1 and EZH2 in leukemia cells

Abstract:

Objective：To test that if Beta-Arrestin1 could bind with enhancer of Zeste homolog 2（EZH2） in acute lymphoblastic leukemia（ALL） CCRF-CEM and Raji cells. Methods：The protein levels of Beta-Arrestin1 and EZH2 in the leukemia cells were determined by Western blot. The location of Beta-Arrestin1 and EZH2 in the leukemia cells was measured by confocal microscopy. Co-immunoprecipitation（CO-IP） was examined for the binding of Beta-Arrestin1 with EZH2 in leukemia cells. Results：Western blot results showed that b/Beta-Arrestin1 and EZH2 expressed in those three leukemia cells. Confocal data showed the colocaliza－tion of Beta-Arrestin1 and EZH2 in K562，CCRF-CEM and Raji cells. CO-IP assay illustrated that Beta-Arrestin1 bind with EZH2 in three leukemia cells. Conclusion：Beta-Arrestin1 could bind to EZH2 in ALL CCRF-CEM and Raji cells.