Immunogenicity on interferon α-2a and interferon α-2b

Abstract:

Objective：To investigate the immunogenicity and the immunotoxicity of interferon α-2a（IFNα-2a） and interferon α-2b（IFNα-2b） on Kunming mice. Methods：Kunming mice were randomly divided into six groups（eight mice in each group）：positive control group（treated with cyclophosphamide，P group），IFNα-2b high dose group（A group），IFNα-2b low dose group（B group），IFNα-2a high dose group（C group），low dose group（D group） and negative control group（treated with solvent，N group）. Mice were treated by intraperitoneal injection for 5 weeks. The serum concentration of IFNα，antibodies of interferon（Anti-IFN） and the im－munostaining result of alexin C3 were determined to evaluate the immunogenicity effect. In addition，the body weight，organ index，hematologic traits and histopathological examination were determined to evaluate the immunotoxicity effect. Results：For the concen－tration of serum IFN，group A（9.27±0.79） was higher than group C（7.80±1.26）（P=0.000）；group B（7.91±1.09） was higher than group D（5.97±0.98）（P=0.013）. For the concentration of Anti-IFN，group A（7.49±1.30） was lower than group C（10.31±0.69）（P=0.000）； the group B（6.18±1.02） was lower than group D（8.24±0.74）（P=0.013）. For the kidney immunohistochemistry，group A（0.51±0.00） was lower than group C（0.54±0.00）（P=0.000）；group B（0.42±0.00） was lower than group D（0.47±0.00）（P=0.013）. For the weight gain，group A was lower than group C（P=0.015）；group B was lower than group C（P=0.003）. There was no significant difference between the experimental group and the N group when comparing the kidney index，liver index，and spleen index. Levels of WBC，PLT，MCH，and LY in group A，B，C and D were higher than those in the N group，and were lower than those in the group P（P<0.05）. There was no significant difference among the IgA，IgM and IgG in the immunoglobulin test results. There was no significant pathological change in spleen slices in the experimental group. Conclusion：There is significant difference between IFNα-2a and IFNα-2b for immunogenicity. Moreover，there is no significant difference between IFNα-2a and IFNα-2b for immunotoxcity.