Application of gene trap technique in genetic screening of genes related to the invasion and matastasis of hepatocellular carcinoma

Abstract:

Objective：To search for genes related to the invasion and matastasis of hepatocellular carcinoma（HCC） with the gene trap－ping technology. Methods：Firstly，SMMC7721 cells were transfected with capture plasmid pU21 vector and then selected by G418 to set up cell lines. Secondly，transwell assay and wound healing assay were used to detect the invasion and migration abilities of these cell lines. Thirdly，5’-Full RACE，gene cloning experiment and gene sequencing experiments were used to identify what the genes were captured by pU21 vector in these cell lines. Results：About 300 cell lines stably transfected with pU21. Invasion and migration abilities of about 70 cell lines were obviously changed. One cell line，A554 was found that the gene captured by pU21 was long inter-genic non-protein coding RNA 52（LINC00052）. Conclusion：Gene trap technology is useful to find genes related to the invasion and matastasis of HCC.