Preliminary research on drug release in vitro and pharmacokinetics in vivo of pyridostigmine bromide nanoemulsion

Abstract:

Objective：To explore the drug release behaviors in vitro and pharmacokinetics in vivo of pyridostigmine bromide na－noemulsion（PBNE）. Methods：The high performance liquid chromatography was established to investigate the release behaviors in vitro and pharmacokinetic behaivors in vivo. Results：The release behaviors of PBNE in 4 media（pH1.2 HCl，pH6.8 PBS，pH7.4 PBS，pH7.8 PBS） was similar to those of pyridostigmine bromide（PB）. The MRT（0-24 h） of PBNE increased by 0.98 h than PB；the Cmax of PBNE was 1.55 times that of PB；the oral bioavailability of PBNE was 1.82 times that of PB. Conclusion：The release behaviors of PBNE in different release medium are similar in vitro. PBNE can change the pharmacokinetic behaviors in vivo；the oral bioavailabil－ity of PBNE is higher than PB and PBNE has a sustained-release effect in vivo.