Establishment of a model of coronary microembolization in rats by intervention

Abstract:

Objective：To study the feasibility of establishing the coronary microembolization in rats with injecting autologous micro thrombus particles by intervention via right common carotid artery. Methods：The 36 rats were randomly divided into 4 groups：24 hours coronary microembolization of model group（n=10），30 days coronary microembolization of model group（n=10），sham group（n=10） and normal group（n=6）. The rats in the coronary microembolization model group received 0.5 mL suspension of micro thrombus particles into the root of aorta by intervention through the right common carotid artery. The sham group rats were injected the same volume of normal saline. The rats of 24 hours model group and 30 days model group were sacrificed on scheduled dates，respectively. The left ventricular anterior and heart apex were cut into the serial sections at the thickness of 5 μm. HE staining and martius-sarlet-blue staining were used to observe the ingredients of the vascular embolus and the number of posi－tive arteries embolization. Hematoxylin-basic-fuchsin-picric staining was used to study the area of myocardial ischemia. Results：The numbers of positive arteries embolization of the 24 h model group[（47.23±6.20）%]，the 30 days model group[（62.88±15.68）%] were significantly higher than that of the sham group[（6.75±5.23）%] in the HE staining（P<0.008）. There was no signifi－cant difference between the sham group and normal group. The amounts of positive arteries embolization of the 24 hours model group[（46.20±10.41）%] and the 30 days model group[（55.30±16.34）%]were significantly higher than that of the sham group[（8.13±8.80）%] in the martius-sarlet-blue staining（P<0.008）. The area of myocardial ischemia in the 24 h model group[（2.46±1.41）%] and the 30 days model group[（6.52±2.22）%] were significantly higher than that of the sham group[（0.05±0.02）%] in the hema－toxylin-basic-fuchsin-picric staining（P<0.017）. Conclusion：The coronary micro-embolization model of the rat can be built success－fully and economically with intervention.