Protective effects of augmenter of liver regeneration on rhabdomyolysis induced acute kidney injury in rats

Abstract:

Objective：To determine whether augmenter of liver regeneration（ALR） attenuated acute kidney injury（AKI） in rats follow－ing intramuscular injection of glycerol and to investigate the possible mechanism. Methods：The therapeutic effect of recombinant hu－man ALR（rhALR，100 mg/kg） on AKI was investigated in a glycerol（50% glycerol saline，10 mL/kg）-induced rhabdomyolysis model of AKI in rats. Male sprague dawley rats were randomly assigned to normal，AKI，or AKI+ALR groups. Renal function，creatine kinase（CK） and renal histology were to checked to determine the extent of renal damage. The expression of proliferating cell nuclear anti－gen（PCNA） was assayed to evaluate cell proliferation. Malondialdehyde（MDA），superoxide dismutase（SOD） and reduced glutathione（GSH） activities were monitored to evaluate oxidative stress. Results：Glycerol treatment induced renal function impairment（BUN and Scr were both increased），CK increase and structural abnormalities including tubular necrosis，cast formation，brush border loss，and interstitial edema. SOD activity and GSH in the kidney decreased，while MDA in the kidney increased in AKI group. A compensatory increase of PCNA was detected in AKI group. ALR had no detectable effects on normal rat kidneys，but it significantly improved renal function and alleviated pathological damage and oxidative stress in glycerol induced acute injured kidneys（AKI+ALR groups）. Renal expression of PCNA also increased in AKI+ALR group compared with that of AKI group. Conclusion：ALR significantly attenuates glycerol-induced AKI. The renal protective effects are associated with the inhibition of oxidative stress and the promotion of renal tubular epithelial cell proliferation.