Influence of inhibiting C5L2 expression on Th17 function and kidney transplantation acute rejection reaction in mouse model

Abstract:

Objective：To explore the influence of inhibiting C5L2 expression on Th17 function and kidney transplantation acute rejec－tion reaction in mouse model. Methods：The C5L2 interference adenovirus was constructed，and injected into kidney transplantation acute rejection reaction mouse model by tail vein. The change of serum creatinine（Cr） was detected. Th17 related cytokines inter－leukin（IL）-6，IL-17 and tumor necrosis factor（TNF）-γ were detected by enzyme-linked immunosorbent assay（ELSA）. Kidney pathological change was detected after 1 weeks. Results：C5L2 interference adenovirus was constructed and injected into kidney transplantation acute rejection reaction mouse model successfully. In experimental group，the levels of serum Cr decreased，IL-17，IL-6 and TNF-γ were decreased. Conclusion：C5L2 interference adenovirus can inhibit the function of Th17 cells，and can reduce the kidney transplantation acute rejection reaction in mouse model.