Mechanism of cell adhesion molecule-1 inhibits the invasion and metastasis of bladder cancer cells

Abstract:

Objective：To investigate the effect of over or low expression of cell adhesion molecule-1（CADM1） on the malignant be－havior of human bladder cancer T24 cells. Methods：The stable expression cells of Ad-CADM1-T24，Ad-GFP1-T24，Ad-sh1-T24，Ad-GFP2-T24 were established before and were used in this study，and T24 cell was used as control. Western blot was used to detect the cell cycle-related protein p27，cyclinD1，cyclinE1，CDK2；and EMT-related protein E-cadherin，β-catenin，Vimentin；and apopto－sis-related protein caspase-3，bcl-2，bax. MTT was used to detect the cell reproductive capacity；and Transwell invasion was used to detect cell invasion ability. Results：Compared with other cells，in Ad-CADM1-T24 cell line，Western blot results showed that the ex－pressions of apoptosis-related protein caspase-3 and bax were increased，while those of bcl-2 were decreased；the expressions of cell cycle-related protein p27 were increased，while those of cyclinD1，CDK2 and cyclinE1 were decreased；the expressions of EMT-related protein E-cadherin and β-catenin were increased，while those of Vimentin were decreased. And the results of MTT showed that the cell proliferation was significantly inhibited in Ad-CADM1-T24 cell line，with statistically significant differences（P<0.05）. And the results of Transwell invasion showed that the number of cell membranes was significantly reduced in 24 h in Ad-CADM1-T24 cell line，with statistically significant differences（P<0.05）. Conclusion：Overexpression of CADM1 can significantly inhibit the invasion and metastasis of bladder cancer cells，the mechanism may be associated with inhibition of cell proliferation and EMT and promoting apoptosis.