Establishment and analysis of nasopharyngeal carcinoma cells CNE-1 with DEPDC1 stable silencing

Abstract:

Objective：To investigate the role of DEP domain containing 1（DEPDC1） in the pathogenesis of nasopharyngeal carcinoma. Methods：The expression level of DEPDC1 in archival tissue samples from nasopharyngeal carcinoma patients and healthy individuals was examined by quatitative RT-PCR and immunohistochemical assay. The nasopharyngeal carcinoma cell line CNE-1 with stable suppression of DEPDC1 expression was established by short hairpin RNA，and their proliferative capability and cell cycle status were separately assessed by CCK-8 assay and FACS assay. Additionally，their migratory and invasive abilities were detected using wound healing assay，Transwell migration and invasion assays. Results：The expression level of DEPDC1 in the tumor tissues（0.699±0.521） was significantly higher than that in the normal tissues（0.408±0.183）（P＜0.05）. Suppression of DEPDC1 in CNE-1 cells revealed significant inhibition of cellular proliferation as well as G2/M phase arrest. Depletion of DEPDC1 also remarkably attenuated cell motility and invasiveness accompanied by the reduction of Twist1（0.710±0.034） and Vimentin （0.780±0.063）（P＜0.05）. Conclusion：Our findings uncover a novel role for DEPDC1 in cell cycle progression and metastasis of nasopharyngeal carcinoma，and provide a rationale for its potential in the diagnosis and/or therapy of nasopharyngeal carcinoma.