Silencing PRR11 inhibits the migration and invasion of lung cancer A549 cells

Abstract:

Objective：To further investigate the molecular mechanism that proline rich protein 11（PRR11） implicates in migration and invasion in lung cancercells. Methods：PRR11 was down-regulated using siRNA in lung cancer cell A549. The migration and inva－sion abilities was detected using wound healing，Transwell，siRNA，and EMT relative proteins was analyzed by Western blot and IF assay. Results：Our results demonstrated that PRR11 depletion significantly inhibited the migration and invasion abilities in A549 cells using wound healing and Transwell. Furthermore，the expression levels of the Twist1 and N-cadherin were down-regulated in PRR11 depletion significantly than that of control using qRT-PCR，Western blot and IF. Conclusion：PRR11 depletion significantly inhibites the migration and invasion abilities in lung cancer cell line. It suggests that high-level expression of PRR11 enhance the migration and invasion activities in lung cancer，but the molecular mechanism is worth for further study.