REGγ induces epithelial-mesenchymal transition in MDA-MB-231 cell lines

Abstract:

Objective：To study the biological role of REGγ gene in promoting epithelial-mesenchymal transition in MDA-MB-231 cell lines. Methods：Immunohistochemistry was used to detect the expression of REGγ in breast cancer tissues and adjacent tissues. Western blot and RT-PCR were used to detect the expression of REGγ in different human breast cancer cell lines. To construct the stable cell lines with overexpression or interference of REGγ gene，and RT-PCR and cellular immunofluorescence were utilized to de－tect the expression level of epithelial-mesenchymal transformed cell marker respectively. The effects of REGγ on cell migration and invasion were confirmed by scratch test and Transwell respectively. Results：Immunohistochemistry suggested that REGγ was signifi－cantly overexpressed in breast cancer（ χ2=25.390，P=0.000； χ2=12.440，P=0.000）. REGγ induced epithelial-mesenchymal transition and improved the cell invasion（t=22.974，P=0.000）. After interfering with REGγ，epithelial-mesenchymal transition，the cell invasion capacity weakened（t=12.118，P=0.000）. Conclusion：REGγ promotes epithelial-mesenchymal transition in human breast cancer MDA-MB-231 cells and promotes invasion and migration of tumor cells.