Overexpression of OTUD1 in HCT116 cells inhibits cell growth，migration and invasion

Abstract:

Objective：To investigate the effects and mechanism of OTUD1 gene on proliferation and invasion of human colon cancer line HCT116. Methods：Human colon cancer cell lines with lower expression of OTUD1 were selected. The OTUD1 gene in HCT116 cells was over expressed by lentivirus infection technique，and empty vector was used as control. After screening by puromycin，HCT116 cells with stably expressed of OTUD1was constructed. RT-PCR and Western blot were used to detect the transfection effi－ciency. The abilities of migration and invasion in vitro were observed by Transwell assay. The CCK-8 cell proliferation assay was used to detect the proliferation ability. The colony formation ability was measured by colony forming assay. The changes of associated molecular level was detected by Western blot to predict its possible mechanism. Results：The expression of OTUD1 in HCT116 cells was the lowest in the 6 human colon cancer cell lines. HCT116-OTUD1 cells with stably-expressed OTUD1 were successfully estab－lished. Compared with that of control group，the migration and invasion ability of HCT116-OTUD1 cells was decreased（P=0.000），the proliferation ability was inhibited（P=0.004），the clone formation ability was decreased（P=0.017），EDU level performed by flow cytometry was down-regulated（P=0.000）. Furthermore，the EMT-associated molecule E-cadherin was up-regulated，while Vimentin was down-regulated，and the proliferation associated molecule p-AKT and p-ERK were down-regulated. Conclusion：OTUD1 might promote HCT116 cells proliferation，migration and invasion.