Inhibition of KIAA1456 on subcutaneous xenografts of human epithelial ovarian carcinoma in nude mice

Abstract:

Objective：To investigate the effect of KIAA1456 on the growth of subcutaneous xenografts of human epithelial ovarian cancer cell line HO-8910PM in nude mice. Methods：Fifteen SPF female BALB/c-nu nude mice were randomly divided into three groups：blank group（PM），negative control group[PM（NC）]，experimental group[PM（KIAA1456）]. The human ovarian cancer cell line HO8910PM，the negative control group HO8910PM（NC） and the cell line HO8910PM over expression KIAA1456[HO8910PM（KIAA1456）] were inoculated respectively into subcutaneous of each group to establish subcutaneous xenografts model in nude mice. After the tumor was successfully formed，the PBS buffer，venous fluid LV-NC and LV-KIAA1456 were respectively intratumoral injected in three groups for every four days. The nude mice were killed at the 28th day. The tumor growth curve was drawn and the tumor inhibition rate was calculated. The expression level of KIAA1456 mRNA and protein in xenografts was detected by Real-time fluorogenic quantitative PCR and Western blot. The expression of ki-67 and PCNA was detected by immunohistochemistry. Results： The expression of KIAA1456 in the HO8910PM（KIAA1456） xenografts group was significantly higher than that in the negative con－trol group and blank control group（P=0.000）. The volume of subcutaneous xenografts in HO8910PM（KIAA1456） group was signifi－cantly smaller than that in the negative control group and blank control group（P=0.000）. The expression of ki-67 and PCNA in the HO8910PM（KIAA1456） group was significantly lower than that in the negative control group and the blank control group，with statis－tically significant differences（P=0.000）. Conclusion：Overex－pression of KIAA1456 inhibits the growth of human epithelial ovarian cancer cell HO8910PM in nude mice subcutaneously，which is expected to be a new target for oncogene therapy.