miR-130b-3p；糖尿病肾病；靶点预测；信号通路；富集分析

Abstract:

Objective：To predict miR-130b-3p target genes by bioinformatic analysis and enrichment analysis for its molecular func－tion，biological process and signal pathway，so as to provide a theoretical basis for the further study of miR-130b-3p associated with diabetic nephropathy. Methods：Database of target gene was retrieved from the NCBI and miRbase database. The sequences of miR-130b-3p were analyzed with Vector NTI，and miR-130b-3p target genes were predicted using TargetScan，picTar，RNA22，PITA and miRanda. Functional enrichment analysis of target genes and signal pathway enrichment analysis were carried out using DAVID and KEGG database. Results：The sequence of miR-130b-3p was highly conserved among various species. GO analysis showed that miR-130b-3p was mainly enriched in nucleoplasm and cytosol. Molecular function was mainly enriched in protein binding and transcription factor activation. Classic miR-130b-3p was enriched in seven signal transduction pathways in KEGG database，including TGF-β，AMPK and endocytosis. The disease enrichment of target genes was highly relevant to diabetic nephropathy. Conclusion：The function and signaling pathway of miR-130b-3p target genes are closely related with the development and progression of diabetic nephropathy.