Expressions of FcεRI and CD63 in eosinophils of patients with allergic rhinitis

Abstract:

Objective：To investigate the expression of FcεRI and CD63 in blood eosinophils from patients with allergic rhinitis（AR）. Methods：Peripheral blood samples were collected from AR patients and healthy controls（HCs），then incubated with crude extracts of artemisia pollen and dust mite. Expressions of FcεRI and CD63 in blood eosinophils were detected by flow cytometry. Results：With－out challenge，it was showed that the ratio of eosinophils in AR patients[0.92%（0.70% to 1.64%）] was increased（Z=-2.050，P=0.041） when compared with that of HCs[0.71%（0.43% to 0.97%）]. Percentage of FcεRI+ eosinophils and mean fluorescence intensity（MFI） of FcεRI expression on eosinophils in the blood of AR patients[6.19%（3.05% to 8.19%）；1 462（1 164 to 1 720）] were enhanced（Z=-2.102，P=0.036；Z=-2.777，P=0.007） when compared with those of HCs[3.16%（1.55% to 4.53%）；808（524 to 1 308）]，respectively. MFI of CD63+ eosinophils in AR patients[2 031（1 725 to 3 338）] was higher（Z=-3.192，P=0.002） than that of HCs[1 491（1 297 to 1 613）]. Artemisia pollen at 0.1 μg/mL[2 618（2 131 to 3 315）] and 1.0 μg/mL[3 108（2 317 to 3 792）] induced upregulation of MFI of CD63+ eosinophils in the blood of AR patients. Conclusion：FcεRI and CD63 are likely to play key roles in AR. Allergen-specific eosinophils challenge test to detect the expression of CD63 may be a supplement experiment for diagnosis of AR.