Effect of Wnt/?茁 -catenin signaling pathway on the repair of orthodontic root resorption

Abstract:

Objective：To investigate the clinical efficacy of Wnt/β-catenin signaling pathway in the repair of movable root resorption. Methods：①The rat model of inflammatory root absorption was established by exerting large orthodontic force，and the research objects were randomly divided into the LiCl group，the DKK1 group and the control group，with 10 rats in each group. Each root surface lacu－na volume change and the changes of bone mineral density were compared by Micro-CT scanning. ②After scanning，the rats in three groups were randomly executed，and the expression of Axin2，beta-catenin，BMP-2，BSP and CAP in the periodontal tissues were de－tected by immunohistochemistry. Results：①In 0 day of repair，the lacunar volume difference of root surface was of no statistical sig－nificance（P>0.05）. After 2 weeks，the repaired volume of root in LiCl group and control group increased significantly（P<0.05），while that of the DKK1 group decreased. The indicators of the three groups changed obviously and the differences were significant（P<0.05）. ②In 0 day of repair，the positive expression levels of Axin2，β-catenin，BMP-2，BSP，and CAP in the three groups had no obvious difference（P>0.05）. On the 3rd day of repair，the expression levels of BMP-2，BSP，and CAP in the three groups had significant dif－ference，and the β-catenin expressions in LiCl group and DKK1 group were significantly different（P＜0.05）. Seven days after repair，the expression levels of Axin2，β-catenin，and BMP-2 BSP and CAP in the LiCl group were statistically higher than those of the DKK1 group and control group（P<0.05）. Conclusion：Through regulating the expressions of Axin2，β-catenin，BMP-2，BSP and CAP，the Wnt/β-catenin signaling pathway can accelerate the root re－sorption lacunaes volume to promote the recovery of trabecular bone structure，and accelerate the repair of moving tooth root resorption in rats.