Investigation of the role of H3K79me2，3 on mitotic progression in osteosarcoma cells

Abstract:

Objective：To determine the H3K79me2，3 level in osteosarcoma cells during mitosis and to investigate its potential influence on mitotic progression. Methods：Western blot was used to detect the H3K79me2，3 level in osteosarcoma cells after mitotic synchro－nization. Time-lapse imaging was performed with a laser confocal fluorescence microscope to monitor the mitotic progression of U2OS cells with or without inhibitor EPZ5676. Results：The H3K79me2，3 level in osteosarcoma cells declined in the prometaphase and then increased gradually with mitosis exit. Depletion of H3K79me2，3 and the following increase caused an increase in the percentage of chromosome missegregation，with no significant effect on the kinetics of mitosis exit（χ2=4.99，P=0.026）. Conclusion：The H3K79me2，3 level shows variations during mitosis in osteosarcoma cells and is related to the regulation of chromosome segregation.