Effect of 14，15-DHET on the expression of endothelial nitric oxide synthase in bovine aortic endothelial cells

Abstract:

Objective：To investigate the effect of 14，15-DHET on the expression of endothelial nitric oxide synthase（eNOS） in bovine aortic endothelial cells（BAECs） and angiogenesis. Methods：According to the concentration of 14，15-DHET，BAECs were divided into 0（control group），0.1，0.5，1，and 5 μmol/L groups，with a stimulation time of 24 hours；according to the time gradient，BAECs were divided into 0（control group），4，8，12，and 24 hours groups，with a stimulation concentration of 5 μmol/L. The expression of eNOS was measured. Human umbilical vein endothelial cells（HUVECs） were divided into control group and treatment group，with a con－centration of 14，15-DHET of 0 and 5 μmol/L，respectively，and the effect of 14，15-DHET on angiogenesis was observed after 24 hours of stimulation. Results：As for concentration gradient，the 0.1，0.5，1，and 5 μmol/L 14，15-DHET groups had an expression level of eNOS protein in BAECs of 1.456±0.008，2.202±0.045，3.931±0.170，and 4.588±0.179，respectively，which was significantly higher than that in the control group（P<0.05）. As for time gradient，the 4，8，12，and 24 hours groups treated with 5 μmol/L 14，15-DHET had an expression level of eNOS protein in BAECs of 1.521±0.021，1.922±0.061，3.642±0.173，and 4.813±0.134，respec－tively，which was significantly higher than that in the control group（P <0.05）. After HUVECs were stimulated by 5 μmol/L 14，15-DHET，the treatment group had significantly longer total tubular length and total tubular branching length than the con－trol group（13 715.000±448.092 and 12 538.000±574.182 vs. 10 426.000±950.537 and 8 672.000±1 025.486，P=0.043 and 0.035）. Conclusion：The results of this study show that 14，15-DHET can increase the expression of eNOS in BAECs and promote angiogenesis.