In vitro release and in vivo gastrointestinal absorption of curcumin ethosomes

Abstract:

Objective：To investigate the in vitro release characteristics and in vivo gastrointestinal absorption of curcumin ethosomes （CMET）. Methods：Curcumin（CM） group，curcumin liposome（CMLP） group，and CMET group were established in this study. The dynamic dialysis method was used to evaluate in vitro release characteristics. A rat model of in vivo single-pass perfusion was estab－lished and CM，CMLP，and CMET were administered，respectively，to investigate the absorption of CM in the stomach and intestinal segments. Results：The cumulative release rates of CMET in the release media（pH 1.2 HCl，pH 6.8 PBS） were （87.77±0.20）% and （84.72±0.53）%，respectively，which were 2.25 and 2.26 times those of CM，respectively，and the release curves were consistent with the Weibull model. The absorption rate constants（Ka） of CMET in the duodenum，the jejunum，the ileum，and the colon were 11.28×10-2，5.24×10-2，6.12×10-2，and 5.93×10-2 L/min，respectively，which were 1.9，1.1，1.6，and 2.6 times those of CM；the effective permeability（Peff） values of CMET in these four intestinal segments were 9.15×10-3，4.21×10-3，5.11×10-3，and 6.41×10-3 cm/s，respectively，which were 2.4，1.3，1.8，and 3.2 times those of CM. The statistical analysis showed that there were significant differences between CMET and CM in Ka in the duodenum，the jejunum，the ileum，and the colon（P=0.006，0.007，0.001，and 0.004，respectively） and Peff in these four intestinal segments（P=0.000，0.028，0.000，and 0.005，respectively）. Conclusion：CMET can improve the in vitro release behavior of CM and promote the absorption of CM in the small intestine.