Abstract:Objective: To investigate the effect of anti-Lingo-1 antibody on oligodendrocytes in the hippocampal dentate gyrus (DG) in rats with depression and cognitive impairment. Methods: After 1 week of adaptive feeding, 70 clean male Sprague-Dawley rats aged 4-6 weeks were randomly divided into control group with 10 rats and model group with 60 rats. A rat model of depression with chronic unpredictable stress (CUS) was established for 4 weeks. The sucrose preference test was used to screen out 23 model rats, and then these rats were randomly divided into CUS groups with 12 rats and anti-Lingo-1 group with 11 rats. The Morris water maze was used to assess the behavioral level of the control group, the CUS group, and the anti-Lingo-1 group, and the stereological method was used to calculate the number of oligodendrocytes in the hippocampal DG for all groups. Results: After 4 weeks of CUS stimulation, compared with the control group, the CUS group had a significant reduction in the number of platform crossings on day 6 (2.4±0.2 vs 3.4±0.2, F = 20.049, P = 0.004); after 3 weeks of anti-Lingo-1 antibody treatment, the anti-Lingo-1 group had a significant increase in the number of platform crossings compared with the CUS group (4.3±0.3 vs 2.4±0.2, F = 20.049, P = 0.000). The stereological results showed that compared with the control group, the CUS group had a significant reduction in the number of oligodendrocytes in the hippocampal DG [(3.1±1.3)×104 vs (4.9±1.1)×104, F = 8.747, P = 0.026); compared with the CUS group, the anti-Lingo-1 group had a significant increase in the number of oligodendrocytes in the hippocampal DG [(6.1±1.1)×104 vs (3.1±1.3)×104, F = 8.747, P = 0.001). Conclusion: Anti-Lingo-1 antibody can improve cognitive impairment in rats with depression. The effect of anti-Lingo-1 antibody on oligodendrocytes in the hippocampal DG in rats with depression and cognitive impairment might be one of the important structural bases for anti-Lingo-1 antibody in improving cognitive impairment in rats with depression.
