Inhibitory effect of RGS2 on inflammation after intracerebral hemorrhage in rats

Abstract:

Objective：To investigate the inhibitory effect of regulator of G-protein signaling 2（RGS2） on inflammation after intracere－bral hemorrhage（ICH） in rats. Methods：Sixty-nine SD rats were divided into sham-operation group，ICH group，negative control lentivirus+ICH group，RGS2 overexpression lentivirus+ICH group，and time window group. Twelve SD rats were assigned to each of the first four groups and three SD rats to each of subgroups under the time window group，consisting of normal，ICH6h，ICH12h，ICH24h，ICH48h，ICH72h，and ICH7d. A rat model of ICH was established by injecting type VⅡ collagenase into the basal ganglia. Neurological function was scored on 3 days after model establishment. The wet-dry weight method was used to measure brain wa－ter content. Western blot and enzyme-linked immunosorbent assay were used to determine the content of tumor necrosis factor-α（TNF-α） and interleukin-1β（IL-1β） in the brain tissue around the hematoma. The immunofluorescence staining method was used to detect the expression of myeloperoxidase（MPO） around the lesion. Results：①The expression of RGS2 increased from （0.5792±0.0198） after ICH，reached a peak level of （0.809 3±0.076 2） at 12 hours after surgery，then decreased，and returned to a normal level of （0.600 0±0.083 0） on 7 days after surgery. ②Compared with the ICH group，the RGS2 overexpression lentivirus+ICH group had a significantly reduced brain water content and a significantly improved neurological function score on 3 days after surgery（0.794 0±0.001 5 vs. 0.804 7±0.003 4；7.429 0±0.976 0 vs. 11.570 0±2.070 0）. ③The expression of TNF-α and IL-1β around the hematoma on 3 days after surgery was significantly lower in the RGS2 overexpression lentivirus+ICH group than in the ICH group（1.407±0.055 vs. 1.743±0.179；0.762±0.046 vs. 0.917±0.085）. The A values of TNF-α and IL-1β were lower in the RGS2 overexpression lentivirus+ICH group than in the ICH group（32.92±1.13 vs. 40.38±0.99；32.84±1.08 vs. 39.29±1.39）. ④The RGS2 over－expression lentivirus+ICH group had significant lower expression of MPO around the lesion than the ICH group and the negative control lentivirus+ICH group. Conclusion：RGS2 inhibits the expression of TNF-α，IL-1β，and MPO around the hematoma after ICH in rats，which can reduce brain edema，improve neurological function，and inhibit inflammation after ICH.