Significance of SPRY1 gene in senescence of keratinocytes

Abstract:

Objective：To investigate the expression and significance of SPRY1 gene in the senescence of normal human epidermal keratinocytes（NHEKs） and immortalized keratinocytes（HaCaT cells） from individuals of different ages. Methods：A total of 120 nor－mal skin specimens were collected from individuals of different ages and divided into juvenile group（<18 years），youth group（18-44 years），middle-aged group（45-59 years old），and elderly group（≥60 years），with 30 specimens in each group. Immunohistochem－istry，real-time PCR，and Western blot were used to compare the localization and expression of SPRY1 in keratinocytes between dif－ferent age groups. HaCaT cells were treated with 200 μmol/L H2O2 to establish a model of cellular senescence. Real-time PCR and Western blot were used to measure the expression of SPRY1 in the groups of normal and senescent HaCaT cells. Results：SPRY1 was mainly expressed in the spinous and granular layers of NHEKs and was distributed in the cytoplasm. The mRNA and protein expres－sion of SPRY1 in keratinocytes gradually increased with the increase in age（P<0.05）. Senescence of HaCaT cells was observed after H2O2 treatment. The mRNA and protein expression of SPRY1 was significantly upregulated in senescent HaCaT cells（P<0.05）. Con－clusion：There is an increase in the expression of SPRY1 gene in senescent NHEKs and senescent HaCaT cells，suggesting that the SPRY1 gene is involved in the regulation of keratinocyte senescence.