Effect of negative pressure suction and iodophor washing on biofilm in diabetic foot wounds

Abstract:

Objective：To explore the effect of biofilm on wound healing in diabetic foot（DF） patients and preliminary intervention. Methods：Patients with DF who were hospitalized from August 2016 to August 2018 and received negative pressure suction and iodophor washing were selected for bacterial culture of secretion specimens isolated from debridement during treatment. Gram staining and alcian blue staining were used to observe the basal tissues of the wound in some cases under optical microscope，and the basal tissues of the same wound were also observed under transmission electron microscopy. All data were entered into SPSS 24.0 software for statistical analysis. Results：The results of positive bacterial culture had a positive rate of 58.3%，and Staphylococcus aureus，Ente－rococcus faecalis，and Staphylococcus epidermidis were the most common bacteria. Bacterial biofilms were observed under both op－tical and transmission electron microscopes. After the intervention of negative pressure suction and iodophor washing，the healing rate was 68.3% after 30 days of hospitalization. The median duration of treatment for DF was 20 days（interquartile range：9-35 d）. The mean white blood cell count was （6.920±3.149）×109/L after 30 days of intervention. Conclusion：The suspected substance was con－firmed as bacterial biofilm by optical and transmission electron microscopy in DF wound infection. Negative pressure suction plus iodophor washing can reduce the chance of survival of bacterial biofilm in wounds.