Differential expression profiles of exosomal microRNAs in ovarian endometriosis based on the microarray technique

Abstract:

Objective：To investigate the expression profile of exosomal microRNAs（miRNAs） in ovarian endometriosis（OEM）. Methods：The microarray technique was used to analyze the exosomal miRNAs of eutopic endometrial stromal cells in three patients with OEM and 3 healthy controls，in order to screen out the differentially expressed miRNAs. Results：A total of 12 differentially expressed microRNAs were screened out；hsa-mir-6829-5p，hsa-mir-5188，hsa-mir-4430-hsa-mir-584-5p，hsa-mir-4485-5p，and hsa-mir-4257 were up-regulated in the case group，while hsa-mir-188-5p，hsa-mir-4741，hsa-mir-4516，hsa-mir-1229-5p，hsa-mir-7150，and hsa-mir-5787 were downregulated. Target gene prediction，gene ontology analysis，and pathway analysis were performed for exosomal miRNAs with significant differential expression，and the results showed that most of the biological functions of target gene aggregation were involved in the regulation of biological progression. Conclusion：There are differentially expressed exosomal miRNAs between OEM patients and healthy controls. The differentially expressed miRNAs have high specificity and can help to further clarify the pathogenesis of OEM and search for new diagnostic markers.