patients with intrauterine adhesions

Abstract:

Objective：To examine the expression of zinc finger protein Snail and nuclear factor kappa B（NF-κB） in the endometrium of patients with intrauterine adhesions（IUAs）. Methods：Endometrial tissues were collected by hysteroscopy from 34 IUA patients（IUA group） and 19 non-IUA patients（control group）. The mRNA and protein expression levels of NF-κB，Snail，E-cadherin，and Vimentin were determined using real-time quantitative PCR，immunohistochemistry，and Western blot. Results：The IUA group had significantly increased expression of NF-κB（2.478±0.279 vs. 1.459±0.323，P=0.013），Snail（2.801±0.502 vs. 1.218±0.313，P= 0.005），and Vimentin（3.581±0.650 vs. 1.756±0.260，P=0.006），but significantly reduced expression of E-cadherin（1.324±0.617 vs. 2.784±0.647，P=0.023），as compared with the control group. Conclusion：NF-κB and Snail may be involved in the development and progression of IUA.